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c1000 touch thermal cycler  (Bio-Rad)


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    Bio-Rad c1000 touch thermal cycler
    C1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 22406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx96+c1000+touch+thermal+cycler+real+time+system/CFX96+Touch+Deep+Well+Real-Time+PCR+Detection+System/pm42833467-85-24-28
    Average 96 stars, based on 22406 article reviews
    c1000 touch thermal cycler - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Co-carriage of respiratory tract bacterial pathogens among under-5 children with pneumococcal carriage in Peninsular Malaysia
    Article Snippet: Nucleic acids were extracted from all culture-positive samples using Monarch ® Genomic DNA Purification Kit (New England Biolabs, USA) directly from the original NP-STGG media following the manufacturer’s instructions. .. A commercial multiplex qPCR kit, AllplexTM Respiratory Panel 4 (Seegene, Republic of Korea) was used to detect seven common respiratory bacterial pathogens: Chlamydophila pneumoniae (CP), Legionella pneumophila (LP), M. pneumoniae (MP), B. pertussis (BP), B. parapertussis (BPP), SPN, and HI on the BioRad CFX96 C1000 Touch Thermal Cycler Real-Time system, according to the manufacturer’s protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Co-carriage of respiratory tract bacterial pathogens among under-5 children with pneumococcal carriage in Peninsular Malaysia
    Article Snippet: Nucleic acids were extracted from all culture-positive samples using Monarch ® Genomic DNA Purification Kit (New England Biolabs, USA) directly from the original NP-STGG media following the manufacturer’s instructions. .. A commercial multiplex qPCR kit, AllplexTM Respiratory Panel 4 (Seegene, Republic of Korea) was used to detect seven common respiratory bacterial pathogens: Chlamydophila pneumoniae (CP), Legionella pneumophila (LP), M. pneumoniae (MP), B. pertussis (BP), B. parapertussis (BPP), SPN, and HI on the BioRad CFX96 C1000 Touch Thermal Cycler Real-Time system, according to the manufacturer’s protocol. ..

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published. ..

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Sarcomere shortening was recorded as described previously.19 Intracellular cAMP levels were assessed in isolated cardiomyocytes (Direct cAMP ELISA kit Enzo Life Sciences, Inc). mRNA and Protein Quantification Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published.9 Protein lysates of either human heart tissue or isolated murine cardiomyocytes were run on 10% SDS-PAGE and blotted onto nitrocellulose membranes. .. For protein quantification the following specific primary antibodies were used: CaMKIIδ total (R&D Systems, MAB4176), CaMKII pThr286 (Thermo, MA1-047), cTNT (Abcam, ab45932), PLB (Badrilla, Ltd, A01014), PLB pSer16 (Badrilla, Ltd, A010-12), PLB pThr17 (Badrilla, Ltd, A010-13), RYR2 (Sigma-Aldrich, HPA020028), RyR2 pSer2808 (Badrilla Ltd., A010-30), TnI (Abcam, ab47003), TnI pSer23/24 (HyTest, Cat4T45), PDE2 (Santa cruz, sc 17228), PDE3A (FabGennix, PD3A-101AP), PDE4D (FabGennix, PD4-401AP), NPR-A (Abcam, ab14356), NPR-B (FabGennix, PGCB-201AP), NPR-C (Novus Biologicals, NBP1-31365), EEF2 (Abcam, ab40812), GAPDH (Santa-Cruz, sc-365062), CSQ (Thermo, #PA1-913; see Supplemental Material), Antimouse (Sigma Aldrich, A0545), Anti-rabbit (Sigma Aldrich, A3682).

    Reverse Transcription:

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published. ..

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Sarcomere shortening was recorded as described previously.19 Intracellular cAMP levels were assessed in isolated cardiomyocytes (Direct cAMP ELISA kit Enzo Life Sciences, Inc). mRNA and Protein Quantification Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published.9 Protein lysates of either human heart tissue or isolated murine cardiomyocytes were run on 10% SDS-PAGE and blotted onto nitrocellulose membranes. .. For protein quantification the following specific primary antibodies were used: CaMKIIδ total (R&D Systems, MAB4176), CaMKII pThr286 (Thermo, MA1-047), cTNT (Abcam, ab45932), PLB (Badrilla, Ltd, A01014), PLB pSer16 (Badrilla, Ltd, A010-12), PLB pThr17 (Badrilla, Ltd, A010-13), RYR2 (Sigma-Aldrich, HPA020028), RyR2 pSer2808 (Badrilla Ltd., A010-30), TnI (Abcam, ab47003), TnI pSer23/24 (HyTest, Cat4T45), PDE2 (Santa cruz, sc 17228), PDE3A (FabGennix, PD3A-101AP), PDE4D (FabGennix, PD4-401AP), NPR-A (Abcam, ab14356), NPR-B (FabGennix, PGCB-201AP), NPR-C (Novus Biologicals, NBP1-31365), EEF2 (Abcam, ab40812), GAPDH (Santa-Cruz, sc-365062), CSQ (Thermo, #PA1-913; see Supplemental Material), Antimouse (Sigma Aldrich, A0545), Anti-rabbit (Sigma Aldrich, A3682).

    cDNA Synthesis:

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published. ..

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Sarcomere shortening was recorded as described previously.19 Intracellular cAMP levels were assessed in isolated cardiomyocytes (Direct cAMP ELISA kit Enzo Life Sciences, Inc). mRNA and Protein Quantification Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published.9 Protein lysates of either human heart tissue or isolated murine cardiomyocytes were run on 10% SDS-PAGE and blotted onto nitrocellulose membranes. .. For protein quantification the following specific primary antibodies were used: CaMKIIδ total (R&D Systems, MAB4176), CaMKII pThr286 (Thermo, MA1-047), cTNT (Abcam, ab45932), PLB (Badrilla, Ltd, A01014), PLB pSer16 (Badrilla, Ltd, A010-12), PLB pThr17 (Badrilla, Ltd, A010-13), RYR2 (Sigma-Aldrich, HPA020028), RyR2 pSer2808 (Badrilla Ltd., A010-30), TnI (Abcam, ab47003), TnI pSer23/24 (HyTest, Cat4T45), PDE2 (Santa cruz, sc 17228), PDE3A (FabGennix, PD3A-101AP), PDE4D (FabGennix, PD4-401AP), NPR-A (Abcam, ab14356), NPR-B (FabGennix, PGCB-201AP), NPR-C (Novus Biologicals, NBP1-31365), EEF2 (Abcam, ab40812), GAPDH (Santa-Cruz, sc-365062), CSQ (Thermo, #PA1-913; see Supplemental Material), Antimouse (Sigma Aldrich, A0545), Anti-rabbit (Sigma Aldrich, A3682).

    SYBR Green Assay:

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published. ..

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Sarcomere shortening was recorded as described previously.19 Intracellular cAMP levels were assessed in isolated cardiomyocytes (Direct cAMP ELISA kit Enzo Life Sciences, Inc). mRNA and Protein Quantification Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published.9 Protein lysates of either human heart tissue or isolated murine cardiomyocytes were run on 10% SDS-PAGE and blotted onto nitrocellulose membranes. .. For protein quantification the following specific primary antibodies were used: CaMKIIδ total (R&D Systems, MAB4176), CaMKII pThr286 (Thermo, MA1-047), cTNT (Abcam, ab45932), PLB (Badrilla, Ltd, A01014), PLB pSer16 (Badrilla, Ltd, A010-12), PLB pThr17 (Badrilla, Ltd, A010-13), RYR2 (Sigma-Aldrich, HPA020028), RyR2 pSer2808 (Badrilla Ltd., A010-30), TnI (Abcam, ab47003), TnI pSer23/24 (HyTest, Cat4T45), PDE2 (Santa cruz, sc 17228), PDE3A (FabGennix, PD3A-101AP), PDE4D (FabGennix, PD4-401AP), NPR-A (Abcam, ab14356), NPR-B (FabGennix, PGCB-201AP), NPR-C (Novus Biologicals, NBP1-31365), EEF2 (Abcam, ab40812), GAPDH (Santa-Cruz, sc-365062), CSQ (Thermo, #PA1-913; see Supplemental Material), Antimouse (Sigma Aldrich, A0545), Anti-rabbit (Sigma Aldrich, A3682).

    Isolation:

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Sarcomere shortening was recorded as described previously.19 Intracellular cAMP levels were assessed in isolated cardiomyocytes (Direct cAMP ELISA kit Enzo Life Sciences, Inc). mRNA and Protein Quantification Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published.9 Protein lysates of either human heart tissue or isolated murine cardiomyocytes were run on 10% SDS-PAGE and blotted onto nitrocellulose membranes. .. For protein quantification the following specific primary antibodies were used: CaMKIIδ total (R&D Systems, MAB4176), CaMKII pThr286 (Thermo, MA1-047), cTNT (Abcam, ab45932), PLB (Badrilla, Ltd, A01014), PLB pSer16 (Badrilla, Ltd, A010-12), PLB pThr17 (Badrilla, Ltd, A010-13), RYR2 (Sigma-Aldrich, HPA020028), RyR2 pSer2808 (Badrilla Ltd., A010-30), TnI (Abcam, ab47003), TnI pSer23/24 (HyTest, Cat4T45), PDE2 (Santa cruz, sc 17228), PDE3A (FabGennix, PD3A-101AP), PDE4D (FabGennix, PD4-401AP), NPR-A (Abcam, ab14356), NPR-B (FabGennix, PGCB-201AP), NPR-C (Novus Biologicals, NBP1-31365), EEF2 (Abcam, ab40812), GAPDH (Santa-Cruz, sc-365062), CSQ (Thermo, #PA1-913; see Supplemental Material), Antimouse (Sigma Aldrich, A0545), Anti-rabbit (Sigma Aldrich, A3682).

    SDS Page:

    Article Title: CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Article Snippet: Sarcomere shortening was recorded as described previously.19 Intracellular cAMP levels were assessed in isolated cardiomyocytes (Direct cAMP ELISA kit Enzo Life Sciences, Inc). mRNA and Protein Quantification Total RNA was isolated with Trizol and purified using the RN-Easy mini Kit (Quiagen). .. Reverse transcription reaction was conducted with iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using a CFX96 C1000 Touch thermal cycler Real-Time System (Bio-Rad) using the SsoAdvanced Universal SYBR Green and gene-specific primers as previously published.9 Protein lysates of either human heart tissue or isolated murine cardiomyocytes were run on 10% SDS-PAGE and blotted onto nitrocellulose membranes. .. For protein quantification the following specific primary antibodies were used: CaMKIIδ total (R&D Systems, MAB4176), CaMKII pThr286 (Thermo, MA1-047), cTNT (Abcam, ab45932), PLB (Badrilla, Ltd, A01014), PLB pSer16 (Badrilla, Ltd, A010-12), PLB pThr17 (Badrilla, Ltd, A010-13), RYR2 (Sigma-Aldrich, HPA020028), RyR2 pSer2808 (Badrilla Ltd., A010-30), TnI (Abcam, ab47003), TnI pSer23/24 (HyTest, Cat4T45), PDE2 (Santa cruz, sc 17228), PDE3A (FabGennix, PD3A-101AP), PDE4D (FabGennix, PD4-401AP), NPR-A (Abcam, ab14356), NPR-B (FabGennix, PGCB-201AP), NPR-C (Novus Biologicals, NBP1-31365), EEF2 (Abcam, ab40812), GAPDH (Santa-Cruz, sc-365062), CSQ (Thermo, #PA1-913; see Supplemental Material), Antimouse (Sigma Aldrich, A0545), Anti-rabbit (Sigma Aldrich, A3682).



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